The Science · Preparation

Mustard / myrosinase addition

Adding powdered mustard seed, a myrosinase source, to cooked broccoli or glucoraphanin-rich preparations.

What changes

Supplies active enzyme after the original enzyme has been lost to heat.

Compounds affected

EMG crops connected to this

Human research

Human randomized trialBioavailability

Adding 1 g of powdered brown mustard to 200 g of cooked broccoli made sulforaphane more than four times as bioavailable as cooked broccoli alone.

Population
Adults
Participants
12
Amount studied
200 g cooked broccoli + 1 g brown mustard powder
Form
Cooked mature broccoli
Measured
Sulforaphane bioavailability

Keep in mind: Small study. Measured absorption, not a health outcome. Mature broccoli, not sprouts or microgreens.

Okunade O et al. · Molecular Nutrition & Food Research, 2018 · View study

Laboratory & mechanistic research

Lab, cell and animal findings explain how something behaves; they do not show the same result in people.

Lab analysis

Boiling broccoli stopped sulforaphane formation; adding powdered mustard seed afterward restarted it.

Form
Cooked mature broccoli

Keep in mind: Lab measurement on cooked mature broccoli, not sprouts or microgreens.

Ghawi SK, Methven L, Niranjan K · Food Chemistry, 2013 · View study

Keep in mind

Small studies measuring absorption, not health outcomes; tested on cooked mature broccoli or extracts, not fresh sprouts.

Sources: peer-reviewed research and NIH/USDA reference data, labeled by study type. EMG foods are not intended to diagnose, treat, cure or prevent any disease.